Absence of gemin5 from SMN complexes in nuclear Cajal bodies

Table 2 Gemin5 staining is "all or nothing".

mAb	Number of nuclei with gems/CBs	Total number of gems/CBs	Average number of gems/CBs per positive nucleus
MANSMA1	113	290	2.57
MANSIP1A	93	242	2.60
GEM4E	103	257	2.50
GEM5Q	27	65	2.41
GEM6F	114	286	2.51
GEM7E	111	280	2.52

In those rare HeLa cell nuclei that have gemin5-positive gems/CBs, nearly all gems/CBs were positive for gemin5. HeLa cell cultures on glass coverslips were fixed with 50:50 acetone: methanol, dried and labelled individually with gemin mAbs. Only nuclei with positive gem/CB staining were counted.

ISSN: 2661-8850