Figure 12

Model of EHD-dependent and EHD-independent trafficking from the early and recycling endosomes. Membrane-bound receptors and their cargo (such as the transferrin receptor and transferrin, respectively) that are destined for recycling to the cell surface following endocytosis can be internalized into the early endosome (EE) compartments, transferred to the endocytic recycling compartment (ERC) before returning to the cell surface. From siRNA-mediated depletion of EHD proteins and transferrin loading and recycling experiments here and elsewhere (Naslavsky et al. 2006) [16], EHD3 and 4 are hypothesized to regulate transferrin trafficking from the EE to the ERC while EHD1 and 2 regulate transferrin exit from the ERC. Proteins such as Rabenosyn5 and Rab11-FIP2 associate with EHD proteins through EH-NPF interactions and facilitate endosomal recycling.