Figure 2From: Shared as well as distinct roles of EHD proteins revealed by biochemical and functional comparisons in mammalian cells and C. elegansAll human EHD proteins are concurrently expressed in multiple cell lines. Aliquots of 100 μg cell lysates were resolved by 8% SDS-PAGE and subjected to immunoblotting with rabbit anti-peptide antisera raised against specific EHD proteins. Endogenous EHD proteins were detected in each cell lysate. The specificity of the antisera for EHD proteins is shown in Additional File 1 using GFP-fusion proteins. Relative molecular weight (MW) markers are indicated in kiloDaltons (kD). As a loading control, β-Actin was blotted.Back to article page