All human EHD proteins are concurrently expressed in multiple cell lines. Aliquots of 100 μg cell lysates were resolved by 8% SDS-PAGE and subjected to immunoblotting with rabbit anti-peptide antisera raised against specific EHD proteins. Endogenous EHD proteins were detected in each cell lysate. The specificity of the antisera for EHD proteins is shown in Additional File 1 using GFP-fusion proteins. Relative molecular weight (MW) markers are indicated in kiloDaltons (kD). As a loading control, β-Actin was blotted.