Figure 5

Differential subcellular localization patterns of human EHD proteins. (A) HeLa cells were transfected with C-terminal GFP-tagged EHD proteins for 24 h, fixed, mounted and scanned by a confocal microscope equipped with a 100× objective lens. Human EHD1-, 3- or 4-GFP are localized on tubular and vesicular structures in the perinuclear area, while EHD2-GFP is seen only in vesicular structures. Cells expressing EHD2 show microspikes; t, tubule; v, vesicle; m, microspikes. (B) HeLa cells were transfected with C-terminal GFP-tagged EHD ΔEH mutants for 24 h, fixed, mounted and scanned by a confocal microscope equipped with a 100× objective lens. Bar, 10 μm.