Differential colocalization of GFP- and DsRed-tagged EHD proteins co-expressed in HeLa cells. HeLa cells were co-transfected with C-terminal GFP- (green) and DsRed-tagged (red) EHD proteins for 24 h, fixed, mounted and scanned by a confocal microscope equipped with a 100× objective lens. Colocalization is indicated when similar shaped structures appear yellow in the Merge (arrowheads). (A) EHD1-GFP, (B) EHD2-GFP, (C) EHD3-GFP, (D) EHD4-GFP co-transfected with each EHD-DsRed construct. Bar, 10 μm.