Figure 7

C282Y HFE stimulates IL-8 and MCP-1 protein production. C282Y HFE increases IL-8 and MCP-1 production. IL-8 (A) and MCP-1 (B) protein production was measured by ELISA form 24-h cell supernatants from HEK293 cells transfected with EV, N HFE, or C282Y HFE cDNAs with or without treatment of 300 μM TUDCA. Values are expressed as picograms per microgram of total protein. C282Y HFE increased IL-8 and MCP-1 protein expression (*, p = 0.0328 and *, p = 0.0149 respectively) compared to WT HFE. Treatment with TUDCA significantly decreased IL-8 and MCP-1 protein expression (#, p = 0.0235 and #, p = 0.0355 respectively) compared to WT HFE. Assays were performed in triplicate and are representative of at least three separate experiments.