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Figure 4 | BMC Cell Biology

Figure 4

From: Proteomic screen in the simple metazoan Hydra identifies 14-3-3 binding proteins implicated in cellular metabolism, cytoskeletal organisation and Ca2+ signalling

Figure 4

Immunoprecipitation of tubulin from Hydra lysate with anti-14-3-3 antibody. Western blot stained with anti-tubulin antibody. Lane 1 shows tubulin precipitated with anti-14-3-3 antibody from hydra lysates, in lane 2 the anti-14-3-3 antibody was omitted as control for non-specific binding of proteins to the beads, in lane 3 peptide C was added before precipitation with anti-14-3-3-antibody and in lane 4 the lysate was treated with λ-phosphatase before preciptiation with anti-14-3-3 antibody.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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