Figure 5

Co-localisation of Hydra 14-3-3 proteins with tubulin and actin. (a, b) right hand panel shows schematic drawings of an epithelial cell (a) and a nerve cell (b) in hydra macerates that were stained with anti-tubulin (green, left hand panel) and anti-14-3-3 antibodies (second panel from left). Single optical sections after laser scanning confocal microscopy from the indicated regions are shown. Merged images (third panel from left) indicate partial co-localisation of 14-3-3 with tubulin (indicated by arrows); (c-e) single confocal sections of basal parts of hydra cells expressing 14-3-3 HyA-GFP (c) or 14-3-3 HyB-GFP (d) in whole mounts stained with rhodamine-phalloidin (red); e: single section of the apical part of an epithelial cell expressing 14-3-3 HyB-GFP counterstained with rhodamine-phalloidin. In the merged images, white arrows indicate co-localisation of 14-3-3 with actin. Scale bars are 10 μm in c and d, and 5 μm in e.