GSK-3β RNAi inhibits chromosome alignment. DLD-1 cells were transfected with siRNA duplexes designed to repress GSK-3β then analysed by immunoblot and immunofluorescence. (A) Immunoblot showing repression of GSK-3β. (B) Images of interphase cells showing micronuclei following repression of GSK3-β. (C) Scatter plot quantitating cells with micronuclei. The data is derived from four independent experiments, with each symbol representing an individual coverslip from which a minimum of 1,000 cells was counted. (D) Images of mitotic cells showing chromosome alignment defects in GSK-3β deficient cells. Bar = 5 μm. (E) Box plot quantitating abnormal metaphases in control (black bar) and GSK-3β RNAi (white bar) populations, 48 and 72 hours after transfection. The data is derived from three independent experiments. (F) Bar graphs quantifying the tubulin intensity at the spindle midzone. Values represent the mean and s.e.m. derived from at least 5 cells.