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Table 4 Effect of GSK-3 inhibitors on spindle morphology

From: GSK-3 inhibitors induce chromosome instability

Parameter

Control

SB-415286

AR-A014418

1-Aza kenpaullone

CHIR99021

Pole-Pole Distance (μm)

8.39 ± 0.45

9.45 ± 0.37

10.36 ± 0.57

9.06 ± 0.39

8.18 ± 0.33

 

(22)*

(20)

(19)

(20)

(18)

 

-

p > 0.05§

p < 0.05

p > 0.05

p > 0.05

Tubulin Intensity (midzone/cell max)

0.39 ± 0.02

0.21 ± 0.01

0.25 ± 0.01

0.24 ± 0.01

0.26 ± 0.02

 

(15)*

(20)

(19)

(20)

(18)

 

-

p < 0.001

p < 0.01

p < 0.001

p < 0.05

Interkinetochore Distance (μm)

0.91 ± 0.04

1.04 ± 0.03

1.05 ± 0.04

0.93 ± 0.02

0.92 ± 0.02

 

(40, 4)

(33, 4)

(20, 3)

(52, 4)

(41, 4)

 

-

p < 0.05

p > 0.05

p > 0.05

p > 0.05

Bub1 Intensity (Bub1:ACA)

100.00 ± 3.30

42.59 ± 0.85

24.24 ± 0.40

46.20 ± 0.98

68.50 ± 0.79

 

(76, 4)‡

(64, 4)

(116, 3)

(81, 4)

(124, 5)

 

-

p < 0.05

p < 0.001

p < 0.001

p > 0.05

BubR1 Intensity (BubR1:ACA)

100.00 ± 0.40

63.67 ± 0.30

31.49 ± 1.24

89.27 ± 0.75

84.08 ± 0.49

 

(121, 7)‡

(115, 6)

(133, 4)

(176, 6)

(127, 5)

 

-

p < 0.001

p < 0.001

p < 0.001

p < 0.001

  1. Quantitation of spindle length (pole to pole distance), inter-kinetochore distance and kinetochore localisation of Bub1 and BubR1 in DLD-1 cells treated with GSK-3 inhibitors.
  2. * Values in parentheses (Number of cells)
  3. ‡ Values in parentheses (Number of kinetochores, Number of cells)
  4. § Kruskal-Wallis Test (Nonparametric ANOVA), Dunn's Multiple Comparisons Test.