Parameter | Control | SB-415286 | AR-A014418 | 1-Aza kenpaullone | CHIR99021 |
---|---|---|---|---|---|
Pole-Pole Distance (μm) | 8.39 ± 0.45 | 9.45 ± 0.37 | 10.36 ± 0.57 | 9.06 ± 0.39 | 8.18 ± 0.33 |
 | (22)* | (20) | (19) | (20) | (18) |
 | - | p > 0.05§ | p < 0.05 | p > 0.05 | p > 0.05 |
Tubulin Intensity (midzone/cell max) | 0.39 ± 0.02 | 0.21 ± 0.01 | 0.25 ± 0.01 | 0.24 ± 0.01 | 0.26 ± 0.02 |
 | (15)* | (20) | (19) | (20) | (18) |
 | - | p < 0.001 | p < 0.01 | p < 0.001 | p < 0.05 |
Interkinetochore Distance (μm) | 0.91 ± 0.04 | 1.04 ± 0.03 | 1.05 ± 0.04 | 0.93 ± 0.02 | 0.92 ± 0.02 |
 | (40, 4) | (33, 4) | (20, 3) | (52, 4) | (41, 4) |
 | - | p < 0.05 | p > 0.05 | p > 0.05 | p > 0.05 |
Bub1 Intensity (Bub1:ACA) | 100.00 ± 3.30 | 42.59 ± 0.85 | 24.24 ± 0.40 | 46.20 ± 0.98 | 68.50 ± 0.79 |
 | (76, 4)‡ | (64, 4) | (116, 3) | (81, 4) | (124, 5) |
 | - | p < 0.05 | p < 0.001 | p < 0.001 | p > 0.05 |
BubR1 Intensity (BubR1:ACA) | 100.00 ± 0.40 | 63.67 ± 0.30 | 31.49 ± 1.24 | 89.27 ± 0.75 | 84.08 ± 0.49 |
 | (121, 7)‡ | (115, 6) | (133, 4) | (176, 6) | (127, 5) |
 | - | p < 0.001 | p < 0.001 | p < 0.001 | p < 0.001 |