Skip to main content

Table 4 Effect of GSK-3 inhibitors on spindle morphology

From: GSK-3 inhibitors induce chromosome instability

Parameter Control SB-415286 AR-A014418 1-Aza kenpaullone CHIR99021
Pole-Pole Distance (μm) 8.39 ± 0.45 9.45 ± 0.37 10.36 ± 0.57 9.06 ± 0.39 8.18 ± 0.33
  (22)* (20) (19) (20) (18)
  - p > 0.05§ p < 0.05 p > 0.05 p > 0.05
Tubulin Intensity (midzone/cell max) 0.39 ± 0.02 0.21 ± 0.01 0.25 ± 0.01 0.24 ± 0.01 0.26 ± 0.02
  (15)* (20) (19) (20) (18)
  - p < 0.001 p < 0.01 p < 0.001 p < 0.05
Interkinetochore Distance (μm) 0.91 ± 0.04 1.04 ± 0.03 1.05 ± 0.04 0.93 ± 0.02 0.92 ± 0.02
  (40, 4) (33, 4) (20, 3) (52, 4) (41, 4)
  - p < 0.05 p > 0.05 p > 0.05 p > 0.05
Bub1 Intensity (Bub1:ACA) 100.00 ± 3.30 42.59 ± 0.85 24.24 ± 0.40 46.20 ± 0.98 68.50 ± 0.79
  (76, 4) (64, 4) (116, 3) (81, 4) (124, 5)
  - p < 0.05 p < 0.001 p < 0.001 p > 0.05
BubR1 Intensity (BubR1:ACA) 100.00 ± 0.40 63.67 ± 0.30 31.49 ± 1.24 89.27 ± 0.75 84.08 ± 0.49
  (121, 7) (115, 6) (133, 4) (176, 6) (127, 5)
  - p < 0.001 p < 0.001 p < 0.001 p < 0.001
  1. Quantitation of spindle length (pole to pole distance), inter-kinetochore distance and kinetochore localisation of Bub1 and BubR1 in DLD-1 cells treated with GSK-3 inhibitors.
  2. * Values in parentheses (Number of cells)
  3. Values in parentheses (Number of kinetochores, Number of cells)
  4. § Kruskal-Wallis Test (Nonparametric ANOVA), Dunn's Multiple Comparisons Test.