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Table 1 Percent radioactivity recovery in cellular fractions from (1-14C) glucose labeled MCF7 cells

From: Naturally occurring and stress induced tubular structures from mammalian cells, a survival mechanism

 

Lipid-phase

Inter-phase

TCA

Ethanol

Aqueous

Zero Incubation

0.8

5

6

0.02

88

Full Incubation

0.3

6

10

0.07

85

Transform ation

0.5 ± 0.2

12 ± 3

21 ± 3

0.4 ± 0.2

66 ± 6

  1. The lipid-phase is the bottom fraction from the chloroform:methanol extraction of the cells. The inter-phase contains cell debris and proteins, and the TCA fraction contains proteins from precipitation of the aqueous fraction. The ethanol fraction contains carbohydrates from the remaining aqueous fraction in 5 volumes of ice-cold ethanol incubated at -20°C over night. The remaining water-soluble fraction contains unused 14C-glucose.
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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