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Table 1 Percent radioactivity recovery in cellular fractions from (1-14C) glucose labeled MCF7 cells

From: Naturally occurring and stress induced tubular structures from mammalian cells, a survival mechanism

  Lipid-phase Inter-phase TCA Ethanol Aqueous
Zero Incubation 0.8 5 6 0.02 88
Full Incubation 0.3 6 10 0.07 85
Transform ation 0.5 ± 0.2 12 ± 3 21 ± 3 0.4 ± 0.2 66 ± 6
  1. The lipid-phase is the bottom fraction from the chloroform:methanol extraction of the cells. The inter-phase contains cell debris and proteins, and the TCA fraction contains proteins from precipitation of the aqueous fraction. The ethanol fraction contains carbohydrates from the remaining aqueous fraction in 5 volumes of ice-cold ethanol incubated at -20°C over night. The remaining water-soluble fraction contains unused 14C-glucose.