Figure 1
From: Identifying genes preferentially expressed in undifferentiated embryonic stem cells
Diagram of the gene trap vector eGeoN/E+pA. Neo, Neo resistance gene; GFP, cDNA encoding green fluorescent protein; pA, polyA addition signal derived from the gene of bovine growth hormone (bGH); SA, splice acceptor sequence present in intron 2/exon 3 of Bcl-2; I(E), IRES sequence from encephalomyocarditis virus (EMCV); I(N), IRES sequence from the 5' UTR region of the NF-kappaB repressing factor (NRF) gene; 3'LTR and 5'LTR, long terminal repeat regions of the RET vector modified from murine Moloney leukemia virus [23]. Both 3'LTR and 5'LTR are comprised of U5, R and U3. The regulatory sequence in the U3 region of 3'LTR was deleted and replaced with a loxP site which could be recognized by Cre recombinase.