The ER morphology is overall normal in exPKO acinar cells. (A, B) Acinar cells of both wild-type and exPKO pancreata exhibit distended and prototypical ER at a similar frequency (A, P16 wild-type, 3000x; B, P16 exPKO, 7500x). (C) An exPKO acinar cell undergoing cell death (oncosis), P25 exPKO, 2500x. (D) The pancreas of the exPKO mouse (P25) contains infiltrating macrophages. The arrow points to the ER of an acinar cell engulfed by a macrophage. 1500x. Macrophages were not seen in the wild-type pancreas (data not shown). Also see Figure 6 for acid phosphatase staining. (E, F) Images of mutant cells undergoing cell death, 1200x. Note that these still retain the nucleus, which is vacuolated and does not contain chromatin condensation.