Figure 6
From: PERK eIF2 alpha kinase is required to regulate the viability of the exocrine pancreas in mice
exPKO acinar cells die through oncosis. (A, B) Acinar cells in the exPKO pancreas have numerous TUNEL-positive cells (A, P31 wild-type; B, P22 exPKO). Note that the TUNEL staining pattern strikingly differs between the two genotypes. TUNEL, green; DAPI, blue; background, red. (C, D) Adjacent serial sections (3μ) of the exPKO pancreas (P19). Pale pink (oncotic) acinar cells identified by H&E staining (C) correspond to cells positive for ApoE (D); see arrows as an examples of several cells. ApoE, red; DAPI, blue; background, green. (E, F) The KO pancreas has significant leukocyte infiltration (E, wild-type; F, exPKO, P74). Dark purple acid phosphatase staining indicates infiltrated leukocytes including macrophages (see arrows) in F but not E. The background cellular staining is methylene blue.