Figure 4
Comparison of phospho-MLC response in individual cells with cells in contact with other cells. Means and standard deviations (n = 3 replicate experiments) are shown. Number of cells measured is about 150 single cells and 30 cells in pairs. A) Comparison of cell spreading between individuals not in direct physical contact with other cells and pairs of cells contacting each other. Statistical significance (p < 0.05) is indicated by '*'. B) Comparison of the percent inhibition of MLC phosphorylation by Y27632 between individuals not in direct physical contact with other cells and pairs of cells contacting each other. Inset B: comparison of absolute MLC phosphorylation in response to Y27632 in single cells and pairs from a representative experiment (Y-axis scale: RFU × 106).