Figure 7

Measurement of ML phosphorylation in NIH 3T3 cells. A) Quantitative Microscopy measurement of phospho-MLC in the absence or in the presence of 10 μM Y27632 in 3T3 cells, B) corresponding western blotting data and C,D) cell spreading. Error bars indicate standard deviations of 3 replicate experiments. Statistical significance (p < 0.005) is indicated by '**'. * (p<0.05).