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Table 1 RNCF and ρ values

From: The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2

 

-

+ a

ρ value b

ρ value c

control (Fus3-GFP)

1.6 ± 0.19

2.2 ± 0.14

n/a

n/a

Dig1 Δ Fus3-GFP

1.7 ± 0.17

1.8 ± 0.12

0.3323

<0.0001

Dig2 Δ Fus3-GFP

1.6 ± 0.13

1.9 ± 0.16

0.4017

<0.0001

control (Fus3T180AY182A-GFP)

1.6 ± 0.18

2.0 ± 0.14

n/a

n/a

dig1 Δ Fus3T180AY182A-GFP

1.5 ± 0.16

2.0 ± 0.22

0.2722

0.1771

dig2 Δ Fus3T180AY182A-GFP

1.5 ± 0.15

1.9 ± 0.16

0.0744

0.3527

control (empty vector)

1.6 ± 0.19

2.2 ± 0.14

n/a

n/a

Msg5 overexpression

1.4 ± 0.13

1.4 ± 0.20

0.0001

<0.0001

Msg5M45A C319A overexpression

1.4 ± 0.12

1.7 ± 0.14

<0.0001

<0.0001

control (NLS-Msg5M45A/NES-

1.7 ± 0.17

1.9 ± 0.20

n/a

n/a

Msg5M45A)

    

NLS-Msg5M45A C319A/NES-Msg5M45A

1.7 ± 0.18

2.1 ± 0.23

0.8380

0.0003

NLS-Msg5M45A/NES-Msg5M45A C319A

1.6 ± 0.18

2.1 ± 0.20

0.0490

0.0003

  1. aRNCF ± s.d. 3 h after pheromone treatment; bρ value when compared to RNCF of untreated control; cρ value when compared to RNCF of pheromone-treated control. n = 25 for all experiments. See additional file 1 for histograms used to calculate the RNCF values for Table 1.
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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