Table 3 Plasmids used in this study
Plasmid | Marker | Source |
|---|---|---|
pIH4.1 | URA3 CEN FUS3-GFP | [52] |
pIH4.1AA | URA3 CEN FUS3T180AY182A-GFP | [52] |
YCplac22/FUS3-GFP (AOB117) | TRP1 CEN FUS3-GFP | [52] |
YCplac22/FUS3T180AY182A-GFP (TBB182) | TRP1 CEN FUS3T180AY182A-GFP | [52] |
pRS316CG/MSG5-GFP (HKB101) | URA3 CEN MSG5-GFP | [52] |
YCplac111/GAL1-MSG5 (HKB100) | LEU2 CEN GAL1-MSG5 | [52] |
YCplac22/GAL1-MSG5 (AEB101) | TRP1 CEN GAL1-MSG5 | [52] |
YCplac111/GAL1-MSG5C319A (TBB109) | LEU2 CEN GAL1-MSG5C319A | This study |
YCplac22/GAL1-STE12 (TBB157) | TRP1 CEN GAL1-STE12 | This study |
YCplac22/NLS-MSG5M45A (TBB172) | TRP1 CEN NLS-MSG5M45A | This study |
YCplac22/NES-MSG5M45A (TBB174) | TRP1 CEN NES-MSG5M45A | This study |
YCplac33/NLS-MSG5M45A (TBB181) | URA3 CEN NLS-MSG5M45A | This study |
YCplac33/NES-MSG5M45A (TBB180) | URA3 CEN NES-MSG5M45A | This study |
YCplac22/NLS-MSG5M45AC319A (TBB178) | TRP1 CEN NLS-MSG5M45AC319A | This study |
YCplac22/NES-MSG5M45AC319A (TBB176) | TRP1 CEN NES-MSG5M45AC319A | This study |
pRS316CG/NLS-MSG5-GFPM45A (TBB171) | URA3 CEN NLS-MSG5M45A-GFP | This study |
pRS316CG/NES-MSG5-GFPM45A (TBB170) | URA3 CEN NES-MSG5M45A-GFP | This study |
YEplac181/PTP2-GFP | LEU2 2 μ PTP2-GFP | [84] |
YEplac181/PTP3-GFP | LEU2 2 μ PTP3-GFP | [84] |