| Well | MSC | dMSC |
---|
negative control | | 14.3 ± 2.9 | 16.3 ± 3.7 |
CCN1/CYR61 | lower | 55.5 ± 6.5 | 40.3 ± 4.0 |
| upper&lower | 18.8 ± 4.4 | 15.0 ± 2.9 |
CCN6/WISP3 | lower | 43.5 ± 3.2 | 30.8 ± 3.3 |
| upper&lower | 12.0 ± 2.2 | 13.5 ± 4.2 |
- Checkerboard analysis for migratory effects of CYR61/CCN1 and WISP3/CCN6 with human MSCs and in vitro osteogenic differentiated MSCs (dMSC). Results are presented as absolute migrated cells (mean ± SD of one quadruplicate determination). 2 μg/ml CYR61/CCN1 or 2 μg/ml WISP3/CCN6 were added only to the lower compartment (lower) or to both the upper and lower compartment (upper&lower) of the chemotaxis chamber to eliminate the concentration gradient.