Figure 5

In vitro flow assays. Whole blood from WT (A) or HS1^-/- (B) mice was flowed over collagen at a shear rate of 1000 s^-1 for 4 min. Aggregates were fixed in formalin and imaged using DIC. Flow experiments were also carried out with DiOC₆ labeled platelets (representative image shown in C) and the mean surface area of aggregates was calculated from fluorescence images (D). Means ± SEM are plotted (n = 3). No significant difference was observed between surface area coverage of WT and HS1^-/- platelets (P = 0.669). Scale bars = 20 μM