Figure 5

Distribution of Escapades in Binucleates. Cells expressing GFP-Esc1p and Htb2p-mRFP (ATY2509) were pre-induced, washed and recultured for 8 hrs at 23°C in glucose medium supplemented with latrunculin A to produce binucleate cells, resulting from occasional incorrect orientation of the spindle. A diploid strain was used to facilitate spatial resolution. (A) Confocal z-sections of a binucleate cell show retention of escapades (arrow) in a single nucleus. (B, C) Epifluorescent imaging of binucleate cells. Phase images indicate that the nuclei are contained within the maternal cytoplasm.