Figure 4

Mitochondrial targeting of HCCR-1 requires the D1-2 region. (A) Schematic structure of HCCR-1 deleted mutants which were COOH-terminally linked by the GFP reporter gene. Grey boxes indicate the hydrophobic region. TM boxes indicate the locations of putative transmembrane domain. (B) Expression of deleted mutants in COS-7 cells. COS-7 cells were transfected with the indicated constructs in the expression vectors. Other conditions were same as in Figure 3B.