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Figure 5 | BMC Cell Biology

Figure 5

From: HCCR-1, a novel oncogene, encodes a mitochondrial outer membrane protein and suppresses the UVC-induced apoptosis

Figure 5

HCCR D1-2 mutant was exclusively localized at the mitochondria. COS-7 cells were transfected with HCCR D1-2 (A) and D2-2 (B) which were linked with GFP at its COOH-termini and then incubated for 2 days. The cells were fractionated into the nuclei (lane 1), post-mitochondria (lane 2), and mitochondria (lane3) fractions. Each 10 μg of proteins was separated by SDS-PAGE and analyzed by immunoblotting using antibodies to the GFP epitope (top panel), the mitochondrial protein VDAC-1 (bottom panel).

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