Effects of siRNA-AdipoR1 transfection on the mineralization of MC3T3-E1 cells. Mineralization of MC3T3-E1 was evaluated by von Kossa and Alizarin red stainings. (A) Plate view of von Kossa and Alizarin red stainings in cultured MC3T3-E1. (B) Quantification of Alizarin red staining via extraction with etylpyridinium chloride. The amount of released dye was quantified by microplate reader at 550 nm. Mineralizarion was significantly decreased in siRNA-AdipoR1 treatment compared to the control (*** p < 0.001). The result was the representative of three different experiments.