Effects of an AICAR treatment on the differentiation of MC3T3-E1 cells. AICAR (0.5 mM), a AMP kinase stimulator, was added after the cells reached confluency. Total RNA was collected at 14, 21, 28 days. Col-I (A) and OCN (B) mRNA expression were increased by the AICAR treatment. Results were expressed as fold increase over the control values at 14 days. The result was the representative of three different experiments. (C) ALP activity of MC3T3-E1 cells by an AICAR treatment. ALP activity was evaluated biochemically. ALP activity was increased compared to the control significantly (* p < 0.05, and ** p < 0.01).