Figure 6

Co-localization of GFP-ML1 with MHCII and MHCII transport to the plasma membrane. A) Confocal images of fixed and permeabilized GFP-ML1 cells co-stained to detect MHCII (red) and GFP-ML1 (green) either in the absence of LPS or after 48 hours in 100 μg/ml LPS. Bar is 10 μm. B) Confocal images of RAW264.7, LS9, and LS10 cells stained to detect surface MHCII either in the absence of LPS or after 24 hours in 100 μg/ml LPS. All images were taken using the same exposure and magnification. C) Quantitation of the levels of MHCII at the plasma membrane in the LPS-treated cells shown in panel B. Bars represent standard deviations from at least 60 measurements for each strain. D) Confocal images of fixed and permeabilized RAW264.7, LS9, and LS10 cells co-stained to detect MHCII (red) and LBPA (green) after 24 hours in 100 μg/ml LPS. Bar is 10 μm.