Translocation of FL-Bid to the mitochondria coincides with a loss of cyt-C. (A)Immunoblot analysis of cytosolic and mitochondria-containing pellet fractions after selective plasma membrane permeabilization in cells exposed to Mg2+-free HBS (controls) or NMDA with or without CA-074-Me. The experiment was performed in duplicate with similar results. (B)Immunofluorescence analysis of cyt-C and Bid distribution in sham- and NMDA-exposed rat hippocampal neurons. Cells were fixed 8 h after termination of the exposure. Nuclei were counterstained with Hoechst 33258. Scale bar = 5 μm.