Figure 10

Effect of digitonin extraction on distribution of CLIC1 in T84 cells. Confluent T84 cell monolayers were stained with FITC-wheat germ agglutinin on ice, then either fixed directly (A, B) or following digitonin extraction (C, D). Cells were then stained for CLIC1 and confocal images obtained at 0.3 μm intervals. For both control (A, B) and digitonin extracted (C, D) samples, a red channel image from the focal plain with the most intense CLIC1 staining (panes A and C) and a merged Z-section generated from a 3.5 μm thick slice through the center of the stack of images (panes B and D) are shown.