Figure 5
Digitonin extraction selectively removes soluble CLIC1 from Panc1 and T84 cells. Panc1 cells (A) or T84 cells (B) were grown to confluence. Parallel cultures were untreated (lanes 1 and 3) or extracted with digitonin (lanes 2 and 4) prior to homogenization and preparation of soluble (S) and membrane (M) fractions. Two micrograms of Panc1 soluble protein (A, lanes 1, 2), 8.5 μg of Panc1 membrane protein (A, lanes 3, 4), 2 μg of T84 soluble protein (B, lanes 1, 2), or 10 μg of T84 membrane protein (B, lanes 3, 4) were separated on SDS-PAGE, blotted and probed with AP1089 antibody.