Figure 9

Redistribution of CLIC1 in T84 cells in response to forskolin. Confluent monolayers of T84 cells grown on filters were fixed directly (A) or pretreated with 10 μM forskolin in growth medium for 10 minutes prior to fixation (B). Cells were stained for CLIC1 and Z-section images created from 2 μm thick slices taken through stacks of confocal images taken at 0.2 μm intervals. Two representative Z-sections are shown from each specimen. The culture surface is at the bottom of each image, the apical surface of the cells at the top. Scale bar represents 10 μm.