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Figure 3 | BMC Cell Biology

Figure 3

From: Degradation of the LDL receptors by PCSK9 is not mediated by a secreted protein acted upon by PCSK9 extracellularly

Figure 3

Gel filtration of conditioned medium from HepG2 cells transiently transfected with D374Y- PCSK9 -FLAG plasmid. Conditioned medium from HepG2 cells transiently transfected with D374Y-PCSK9-FLAG plasmid or with empty plasmid were concentrated approximately 10 times and 1 ml was loaded onto a Superdex 200 gel filtration column. Fractions of 2 ml were collected. HepG2 cells were incubated with the different fractions to determine the effect on internalization of LDL by flow cytometry. Fractions containing molecular weight standards are indicated on the x-axis. Aliquotes of fractions 15–21 were also subjected to precipitation of proteins by trichloroacetic acid. The amount of mature D374Y-PCSK9-FLAG in the fractions was determined by western blot analysis using an anti-FLAG antibody as shown in the insert.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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