Figure 5
Spermatid individualization in the control (A-D) and Mer3 (E-I) cysts. Panels A and E illustrate the phase-contrast view of a control or Mer3 cyst, respectively. Panels B and F show the location of the DAPI-stained sperm nuclei. Panels C and G display the sites (arrowheads) and orientation of the actin cones as visualized by FITC-conjugated phalloidin staining. Panels D and H represent merged images. Panel I is an enlarged view of the rectangular area denoted in panel F.