GSK3 inhibitors induced β-catenin nuclear translocation and Gli1 gene expression. hMADS3 cells were maintained in proliferation medium supplemented with 0.5% FCS, then localisation of β-catenin was revealed after treatment with 0.5 μM BIO or 0.5 μM MeBIO for 24 hours. DAPI was used to label nuclei (A). hMADS2 and hMADS3 cells were maintained as in (A) and fold induction in the expression of Gli1 gene was quantified by real-time PCR 24 hours after treatment with 0.5 μM BIO, 0.5 μM MeBio, 20 mM LiCl or 20 mM NaCl or under control condition (Ctr). Bars are the means ± S.E of 2 independent experiments, **: p < 0.01 (B).