Figure 4
From: Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue
Storage of FFPE samples after immunofluorescence labelling. A. 3 μm FPPE section of columnar cell change human breast was stained as described in Figure 2. Images were collected directly after labelling (day 1) and again following 70 days storage of the stained slides at 4°C. B. 3 μm FFPE section of human kidney was stained with anti-collagen IV Ab (red) and mouse anti-alpha smooth muscle actin mAb (green) followed by Alexa555 goat anti-rabbit Ig and Alexa488-conjugated goat anti-mouse IgG2A. Nuclei were counterstained with DAPI (blue). Images were collected directly after labelling (day1) and again following 270 days storage of the stained slides at -20°C. Scale bar, 50 μm.