Table 1 List of antibodies, suppliers and concentrations used
From: Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue
Antigen | Isotype | Suppliers | Concentration |
|---|---|---|---|
E-cadherin | mouse IgG2A | BD Biosciences, #610181 (Oxford, UK) | 0.25 μg/ml |
Ksp-cadherin | mouse IgG1 | Zymed, clone 4H6/F9, #18–7383 (InVitrogen) | 0.4 μg/ml |
collagen IV | rabbit polyclonal | Acris, #R1041 (Herford, Germany) | 5.0 μg/ml |
Ck 8/18 | mouse IgG1 | Novocastra, clone 5D3, #5D3CE (Newcastle upon Tyne, UK) | 0.94 μg/ml |
vimentin | mouse IgG2A | Dako, clone Vim 3B4, #M7020 | 0.25 μg/ml |
ER | rabbit polyclonal | Labvision, clone SP1, #RM-9101-S1 (Runcorn, Cheshire, UK) | 1:400 |
αSMA | mouse IgG2A | Sigma, #A2547 clone 1A4 | 0.88 μg/ml |
Second layer antibody | Company | Concentration | |
Alexa488-conjugated goat anti-mouse IgG2A | Invitrogen, #A21131 | 2.0 μg/ml | |
Alexa488-conjugated goat anti-mouse IgG1 | Invitrogen, #A21121 | 2.0 μg/ml | |
Alexa555-conjugated goat anti-mouse IgG1 | Invitrogen, #A21127 | 2.0 μg/ml | |
Alexa555-conjugated goat anti-rabbit Ig | Invitrogen, #A21429 | 2.0 μg/ml | |
Alexa633-conjugated goat anti-mouse IgG2A | Invitrogen, #A21136 | 2.0 μg/ml | |