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Figure 4 | BMC Cell Biology

Figure 4

From: Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter

Figure 4

Trafficking and internalization of membrane proteins. (a) HeLa cells transiently expressing β1Int-HaloTag were labeled with the HaloTag TMR ligand, then fixed and processed for immunocytochemistry to visualize the endoplasmic reticulum (ER), ER-golgi intermediate complex (ERGIC), or golgi followed by a green secondary antibody. (b) HeLa cells transiently expressing β1Int-HaloTag were labeled with the HaloTag 488 ligand, then fixed and processed for immunocytochemistry to visualize early endosomes (Rab4), late endosomes (Rab11) or cadherin followed by a red secondary antibody. (c) Hela cells transiently expressing β1Int-HaloTag were labeled with the HaloTag 488 ligand and an Alexa Fluor® 594 transferrin conjugate then fixed. Cell images were generated with Olympus FV500 confocal microscope in sequential mode using appropriate filter sets.

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