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Figure 6 | BMC Cell Biology

Figure 6

From: Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter

Figure 6

Cell capture using HaoTag Technolgy. (a) HeLa cells co-expressing β1Int-HaloTag and luciferase were labeled with HaloTag PEG-Biotin. They show specific capture on SA plates compared to labeled cells co-expressing HaloTag and luciferase. Pre-coating plates with HaloTag PEG-Biotin blocks the specific capture. (b) HEK293 cells stably expressing β1Int-HaloTag (top panel) or wildtype HEK293 (bottom panel) were labeled with HaloTag PEG-Biotin. After capture and replate, cells were Tamra labeled and live cell imaging shows β1Int-HaloTag-expressing cells are specifically captured and survive.

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