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Table 2 Plasmids used in this study.

From: A yeast three-hybrid system that reconstitutes mammalian hypoxia inducible factor regulatory machinery

  

Expression from PADH1 promoter

Expression from PMET25 promoter

NAME

VECTOR

Protein

Fragment

MUTATIONS

Protein

Fragment

AD-P564

pGAD-T7

GAL4 AD- HIF

554–576

wt

  

AD-mutP564A

pGAD-T7

GAL4 AD- HIF

554–576

P564A

  

AD-L574A

pGAD-T7

GAL4 AD- HIF

554–576

L574A

  

AD-L562R

pGAD-T7

GAL4 AD- HIF

554–576

L562R

  

AD-P402

pGAD-T7

GAL4 AD- HIF

392–414

wt

  

AD-SH2

pGAD-T7

GAL4 AD- SYK SH2

11–236

wt

  

DB-VHL

pBRIDGE

GAL4 DBD- VHL

63–157

wt

  

E3/DB-VHL

pBRIDGE

GAL4 DBD- VHL

63–157

wt

EGLN3

Full length

E1/DB-VHL

pBRIDGE

GAL4 DBD- VHL

63–157

wt

EGLN1

Full length

DB-ITAM

pBRIDGE

GAL4 DBD- ITAM

45–86

wt

  

LCK/DB-ITAM

pBRIDGE

GAL4 DBD- ITAM

45–86

wt

LCK

Full length

DB-E3

pBRIDGE

GAL4 DBD- EGLN3

Full length

wt

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