Skip to main content

Table 2 Plasmids used in this study.

From: A yeast three-hybrid system that reconstitutes mammalian hypoxia inducible factor regulatory machinery

   Expression from PADH1 promoter Expression from PMET25 promoter
NAME VECTOR Protein Fragment MUTATIONS Protein Fragment
AD-P564 pGAD-T7 GAL4 AD- HIF 554–576 wt   
AD-mutP564A pGAD-T7 GAL4 AD- HIF 554–576 P564A   
AD-L574A pGAD-T7 GAL4 AD- HIF 554–576 L574A   
AD-L562R pGAD-T7 GAL4 AD- HIF 554–576 L562R   
AD-P402 pGAD-T7 GAL4 AD- HIF 392–414 wt   
AD-SH2 pGAD-T7 GAL4 AD- SYK SH2 11–236 wt   
DB-VHL pBRIDGE GAL4 DBD- VHL 63–157 wt   
E3/DB-VHL pBRIDGE GAL4 DBD- VHL 63–157 wt EGLN3 Full length
E1/DB-VHL pBRIDGE GAL4 DBD- VHL 63–157 wt EGLN1 Full length
DB-ITAM pBRIDGE GAL4 DBD- ITAM 45–86 wt   
LCK/DB-ITAM pBRIDGE GAL4 DBD- ITAM 45–86 wt LCK Full length
DB-E3 pBRIDGE GAL4 DBD- EGLN3 Full length wt   
Back to article page

BMC Molecular and Cell Biology

ISSN: 2661-8850

Contact us