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Figure 4 | BMC Cell Biology

Figure 4

From: The CMV early enhancer/chicken β actin (CAG) promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitors

Figure 4

Sustained GFP expression in CCE cells during differentiation into mesoderm is obtained using the CAG promoter. CCE cells were transfected with pCAGIPuro-GFP (A, B) or pCAGGSneo-GFP (C, D) and selected for puromycin or G418 resistance, respectively. FACS analysis was performed on undifferentiated CCE cells (A, C) and cells differentiated to the Flk1 stage (B, D)14–18 days posts transfection and GFP expression was compared in vectors expressing GFP (red) and empty vector (blue) transfected controls.

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