Table 2 Primers used in this study.
Primer | Sequence | Purpose |
|---|---|---|
SDC1for | ATGAGACGCGCGGCGCTC | RTPCR |
SDC1rev | GGCGTAGAACTCCTCCTGC | RTPCR |
SDC2for | GCCTTGATGGCCTGTGTGTC | RTPCR |
SDC2rev | GCATAAAACTCCTTAGTGGG | RTPCR |
SDC3for | CGTGGCTGACGTAAGGACC | RTPCR |
SDC3rev | CTCTAGTATGCTCTTCTGAA | RTPCR |
SDC4for | CGAGAGACAGAGGTCATCGAC | RTPCR |
SDC4rev | TGCGTAGAACTCATTGGTGG | RTPCR |
bactfor | ATGGATGACGATATCGCTGCG | RTPCR |
bactrev | CTAGAAGCATTTGCGGTGCAC | RTPCR |
SDC2ecoRIfor | TATATAGAATTCGTAGGAGCCACATCCCTG | SDC2 cloning |
SDC2ecoRIrev | TATATAGAATTCTTATGCATAAAACTCCTTAGTGG | SDC2 cloning |
SDC4ecoRIfor | TATATAGAATTCGACTGGTTTGCGCTGTTGAA | SDC4 cloning |
SDC4ecoRIrev | TATATAGAATTCTCATGCGTAGAACTCATTGGT | SDC4 cloning |
IRESdelfor | GATATCCGCTAGCGCTACCGGACT | Deletion of CMV promoter from pIRES2-EGFP |
IRESdelrev | ATGCATGGCGGTAATACGGT | Deletion of CMV promoter from pIRES2-EGFP |
GFPfor | TATATAAAGCTTGCCACCATGGTGAGCAAG | GFP cloning |
GFPrev | TATATAGGATCCATGATCTAGAGTCGCGGCC | GFP cloning |