Figure 4

Microtubule Patterns in Interphase Cells. Maximum intensity projections of deconvolved image stacks showing interphase microtubule distributions in wild-type AX-2 and kinesin-null cells (fixed cells imaged by indirect immunofluoresence, using a tubulin antibody). Each row displays four examples of the cell strain indicated on the left. Inserts in the first column show 2X enlargements of the MTOC area. For AX-2, kif4^- and kif11^- cells, a distinctive ring-like appearance of the centrosome can be seen. This feature is less apparent in the kif8^- and kif10^- cells, moreover, a convergence of microtubules into the centrosome in the kif10^- cell is less obvious. Scale bar = 5 μm.