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Table 1 Primers used in the study

From: Application of bioinformatics-coupled experimental analysis reveals a new transport-competent nuclear localization signal in the nucleoprotein of Influenza A virus strain

S. No. Clone Primer No. Sequence Restriction sites
1 NP-FL 35104 5'-cgcgaattc atggcgaccaaaggc EcoRI
   35575 3'-gaccccgggccc attgtcgtactcctc ApaI
2 obpNLS+NC 36823 5'-cgc gaattc atccaaatgtgcaccg EcoRI
   62638 3'-cgcgggccc tttgatcattctgat ApaI
3 obpNLS+NTer 39815 5'-gggctcgag catccaaatgtgc XhoI
   39821 3'-gcggaattc gcgccagattcgtct EcoRI
4 obpNLS+CTer 39820 5'-gggctcgag caagaaaactggagga XhoI
   62638 3'-cgcgggccc tttgatcattctgat ApaI
5 bpNLS-1R 39815 5'-gggctcgag catccaaatgtgc XhoI
   39813 3'-gcgcccggg tcatctcctccactt ApaI
6 bpNLS-1M 39815 5'-gggctcgag catccaaatgtgc XhoI
   39818 3'-gcgcccggg tcatctcatccactt ApaI
7 bpNLS-2R 39814 5'-gcggaattc aggagagaactcatc EcoRI
   62638 3'-cgcgggccc tttgatcattctgat ApaI
8 bpNLS-2M 39819 5'-gcggaattc atgagagaactcatc EcoRI
   62638 3'-cgcgggccc tttgatcattctgat ApaI
9 NPΔ obpNLS 39815 5'-gggctcgag catccaaatgtgc XhoI
   40466 3'-gcggaattc aggatctttccc EcoRI
   39817 5'-gaggaattc caagctaataatggt EcoRI
   62638 3'-cgcgggccctttgatcattctgat ApaI
10 NP41–89 39815 5'-gggctcgag catccaaatgtgc XhoI
   40466 3'-gcggaattc aggatctttccc EcoRI
11 NP122–197 39817 5'-gaggaattc caagctaataatggt EcoRI
   62638 3'-cgcgggccctttgatcattctgat ApaI
12 2bpNLS+NSP6 39820 5'-gggctcgag caagaaaactggagga XhoI
   39821 3'-gcggaattc gcgccagattcgtct EcoRI