Osteogenesis of both mMSCs. MSCs (3 C57BL/10 MSCs and 3 mdx MSCs) were induced in an osteogenic medium, and osteoblast differentiation was indicated by alizarin red staining. Negative control (A), C57BL/10 MSCs (B), and mdx-MSCs (C). The osteocalcin gene was detected in induced osteoblasts by RT-PCR. Lane 1-mdx MSCs, Lane 2-C57BL/10 MSCs, and Lane 3-negative control (D). Lane M, marker; GAPDH, glyderaldehyde-3-phosphate dehydrogenase; OCN, osteocalcin. Scale bar, 100 μm.