Figure 6

Overexpression of mNek1 (1–686) causes the dismantling of the centrosome. Expression of myc-mNek1 (1–686) (red, A) results in loss of centrioles (centrin, green), whereas myc-mNek1 (1–686) (K₃₃R) transfected cells retain centrin foci (B). (C and D) Time course of γ-tubulin foci and/or centrin foci presence in cells transfected with (C) myc-mNek1 (1–686), or (D) myc-mNek1 (1–686; K₃₃R). Cells transfected with myc-mNek1 (1–686) are not observed to retain γ-tubulin foci while lacking centrin foci, indicating that loss of γ-tubulin foci precedes loss of centrin. (E) Representative image of α-tubulin (green), myc-mNek1 (1–686) (red), and γ-tubulin (blue) demonstrating that gross alterations to the microtubule cytoskeleton do not precede γ-tubulin loss in transfected cells. Bar, 10 μm.