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Table 2 S. cerevisiae strains used in this study

From: Mutational analysis of βCOP (Sec26p) identifies an appendage domain critical for function

Strain Genotype Source
RCY269 MAT a ura3-52 leu2-3,112 This lab
RCY3130 MAT α his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 SEC26ΔKANR[pRS316 SEC21 SEC26 pRC2374] This lab
RCY3315 MAT a ura3-52 leu2-3,112 SEC26Δ::sec26FW (F856A W860A)LEU2 This study
RCY3661 MAT a ura3-52 leu2-3,112 SEC26Δ::sec26ts 11D26 (sec26-2, E633D I733T T768P D781V F802L) LEU2 This study
RCY3674 MAT α glo3Δ::KANRhis3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 ResGen
RCY3675 MATaglo3Δ::KANRhis3Δ0/his3Δ0 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 MET15/met15Δ0 ResGen
RCY3676 MAT α gcs1Δ::KANRhis3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 ResGen
RCY3677 MAT agcs1Δ::KANRhis3Δ0/his3Δ0 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 MET15/met15Δ0 ResGen
RCY3782 MAT α glo3Δ::HIS3 his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 This study
RCY3784 MAT a glo3Δ::KAN R his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 met15Δ0 This study
RCY3785 MAT a gcs1Δ::KAN R his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 met15Δ0 This study
RCY3786 MAT a glo3Δ::KAN R his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 This study
RCY3787 MAT a gcs1Δ::KAN R his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 This study
RCY3881 MAT a glo3Δ::HIS3 his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 This study
RCY3882 MAT a gcs1Δ::HIS3 his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 This study
RCY4038 MAT α his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 SEC26Δ::KANRglo3Δ::HIS3 [pRS316 SEC21 SEC26] This study
RCY4233 MAT a his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 gcs1Δ::KANRglo3Δ::HIS3 [pRS316 GLO3-GFP pRC3341A] This study
RCY4236 MAT α his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 gcs1Δ::KANRglo3Δ::HIS3 [pRS316 GLO3-GFP] This study
RCY4238 MAT ahis3Δ0/his3Δ0 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 MET15/met15Δ0 gcs1Δ::KANR/gcs1Δ::KANRglo3Δ::HIS3/glo3Δ::HIS3 [pRS316 GLO3-GFP] This study