Table 2 S. cerevisiae strains used in this study
From: Mutational analysis of βCOP (Sec26p) identifies an appendage domain critical for function
Strain | Genotype | Source |
|---|---|---|
RCY269 | MAT a ura3-52 leu2-3,112 | This lab |
RCY3130 | MAT α his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 SEC26ΔKANR[pRS316 SEC21 SEC26 pRC2374] | This lab |
RCY3315 | MAT a ura3-52 leu2-3,112 SEC26Δ::sec26FW (F856A W860A)LEU2 | This study |
RCY3661 | MAT a ura3-52 leu2-3,112 SEC26Δ::sec26ts 11D26 (sec26-2, E633D I733T T768P D781V F802L) LEU2 | This study |
RCY3674 | MAT α glo3Δ::KANRhis3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 | ResGen |
RCY3675 | MATa/α glo3Δ::KANRhis3Δ0/his3Δ0 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 MET15/met15Δ0 | ResGen |
RCY3676 | MAT α gcs1Δ::KANRhis3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 | ResGen |
RCY3677 | MAT a/α gcs1Δ::KANRhis3Δ0/his3Δ0 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 MET15/met15Δ0 | ResGen |
RCY3782 | MAT α glo3Δ::HIS3 his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 | This study |
RCY3784 | MAT a glo3Δ::KAN R his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 met15Δ0 | This study |
RCY3785 | MAT a gcs1Δ::KAN R his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 met15Δ0 | This study |
RCY3786 | MAT a glo3Δ::KAN R his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 | This study |
RCY3787 | MAT a gcs1Δ::KAN R his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 | This study |
RCY3881 | MAT a glo3Δ::HIS3 his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 | This study |
RCY3882 | MAT a gcs1Δ::HIS3 his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 | This study |
RCY4038 | MAT α his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 SEC26Δ::KANRglo3Δ::HIS3 [pRS316 SEC21 SEC26] | This study |
RCY4233 | MAT a his3Δ0 leu2Δ0 ura3Δ0 met15Δ0 gcs1Δ::KANRglo3Δ::HIS3 [pRS316 GLO3-GFP pRC3341A] | This study |
RCY4236 | MAT α his3Δ0 leu2Δ0 ura3Δ0 lys2Δ0 gcs1Δ::KANRglo3Δ::HIS3 [pRS316 GLO3-GFP] | This study |
RCY4238 | MAT a/α his3Δ0/his3Δ0 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 MET15/met15Δ0 gcs1Δ::KANR/gcs1Δ::KANRglo3Δ::HIS3/glo3Δ::HIS3 [pRS316 GLO3-GFP] | This study |