Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis

Adam, Rosalyn M; Yang, Wei; Di Vizio, Dolores; Mukhopadhyay, Nishit K; Steen, Hanno

doi:10.1186/1471-2121-9-30

BMC Molecular and Cell Biology

Table 1 Proteins identified by tandem mass spectrometry.

From: Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis

(A) Proteins associated with DRMs
DRM proteins			References
Accession #	Score	Description
IPI00006211	175	VAMP (Vesicle-associated membrane protein)-associated protein B and C^a	[42]
IPI00008453	122	Coronin-1C	[26]
IPI00013981	95	YES tyrosine kinase	[25]
IPI00015148	131	Ras-related protein Rap-1b	[40]
IPI00018511	95	Tubulin beta-4q chain	[40]
IPI00022418	285	Splice Isoform 1 of Fibronectin precursor	[26]
IPI00027438	388	Flotillin-1	[16, 25, 39]
IPI00029625	133	Flotillin-2	[16, 29, 39, 40]
IPI00030910	185	GPI-anchored protein p137^a	[43]
IPI00030919	95	Mitogen-activated protein kinase kinase 1 interacting protein 1	[25], [40]
IPI00030939	185	Alpha subunit of GsGTP binding protein	[25]
IPI00216308	2152	Voltage-dependent anion-selective channel protein 1	[44]
IPI00220416	101	Ubiquinol-cytochrome c reductase complex 14 kDa protein	[29, 44]
IPI00221232	106	G-protein gamma-12 subunit variant	[25]
IPI00334190	552	Stomatin-like protein 2	[44]
IPI00337415	81	Guanine nucleotide-binding protein G(i) alpha-1 subunit	[25]
IPI00339269	163	Heat shock 70 kDa protein 6	[40]
IPI00440493	440	ATP synthase alpha chain, mitochondrial precursor	[40]
IPI00554701	72	Ubiquinol-cytochrome c reductase complex 7.2 kDa protein	[29, 44]
IPI00554711	603	Junction plakoglobin	[25]
IPI00641181	143	MARCKS-like 1	[28]
(B) Nuclear proteins shown to exist in other subcellular locations
Nuclear proteins
Accession #	Score	Description	Evidence for shuttling
IPI00000690	87	Splice Isoform 1 of Programmed cell death protein 8, mitochondrial precursor	Yes: [46]
IPI00012048	76	Nucleoside diphosphate kinase A	Yes: [47]
IPI00012442	190	Ras-GTPase-activating protein binding protein 1	Yes: [48]
IPI00016249	91	Fragile X mental retardation syndrome-related protein 1	Yes: [49]
IPI00016250	71	Fragile X mental retardation syndrome-related protein 2	Yes: [49]
IPI00021840	230	40S ribosomal protein S6	Yes: [50]
IPI00023591	158	Transcriptional activator protein PUR-alpha	No:
IPI00024157	114	FK506-binding protein 3	No:
IPI00027096	74	39S ribosomal protein L19, mitochondrial precursor	Yes: UniProt 49406
IPI00027415	171	MLEL1 protein	No:
IPI00028376	92	Mitochondrial import inner membrane translocase subunit TIM8 A	Yes: [51]
IPI00032003	60	Emerin	No:
IPI00042578	83	Similar to heterogeneous nuclear ribonucleoprotein A1 isoform a	Yes: [58]
IPI00165506	125	Polymerase delta interacting protein 2	Yes: [52]
IPI00215637	131	DEAD-box protein 3, X-chromosomal	Yes: [53]
IPI00215780	167	40S ribosomal protein S19	Yes: [54]
IPI00217468	87	Histone H1.5	No:
IPI00219330	206	Splice Isoform 5 of Interleukin enhancer-binding factor 3	Yes: [55]
IPI00328715	216	LYRIC protein	Yes: [56]
IPI00410693	94	Splice Isoform 1 of Plasminogen activator inhibitor 1 RNA-binding protein	Yes: [57]
IPI00456731	123	Similar to Laminin receptor 1	Yes: UniProt P08865

(A) The table illustrates proteins identified in DRM fractions generated using the modified fractionation method that have previously been described as present in or associated with DRMs isolated by other methods. (B) The table lists proteins identified in the modified DRM (TI) fraction that are indicated to be nuclear-localized based on Gene Ontology Cellular Component terminology, but that have been reported to exist in other locations. Proteins that are restricted to nuclei are shaded in grey.
^aLikely DRM-associated.

Back to article page

ISSN: 2661-8850

Contact us

General enquiries: journalsubmissions@springernature.com