Figure 5
From: The HIV Tat protein affects processing of ribosomal RNA precursor
Tat interaction with the fibrillarin-U3 snoRNA complex. Extracts from control flies (WG) (lane 1), and Tat-transgenic flies (Tat-strain) (lane2), following heat-shock treatment. (A) From top to bottom: nuclear lysate probed with anti-fibrillarin antibody; anti-fibrillarin immunoprecipitate probed with anti-fibrillarin antibody; anti-fibrillarin immunoprecipitate probed with anti-Tat; antibody; heavy chain (h.c.) are shown as loading control. (B) From top to bottom: Nuclear lysate probed with anti-Tat; anti-Tat immunoprecipitate probed with anti-Tat antibody; anti-Tat immunoprecipitate probed with anti-fibrillarin antibody; nuclear lysate immunoprecipitated with anti Tat antibody, after RNase treatment, and than probed with anti fibrillarin antibody; antibody heavy chain (h.c.) are shown as loading control. (C) Northern blotting of nuclear extract immunoprecipitated with anti-Tat and probed with U3 specific oligonucleotide. (D) Immunoprecipitation with an unrelated antibody. From top to bottom: nuclear lysate probed with anti Tat antibody; nuclear lysate immunoprecipitated with anti tyrosine hydroxylase (T.H.) and probed with anti Tat antibody; antibody heavy chain (h.c.) are shown as loading control.