Figure 3

NAC effect on viability and NO production in CEES/LPS treated RAW 264.7 cells (NAC post-treatment). Panel A: Macrophages were incubated with 50 or 100 ng/ml of LPS or/and 500 μM CEES (as indicated) for 24 hours and 10 mM NAC was added to the cell culture medium 5 hours after the CEES/LPS application. Cell viability was measured using the MTT assay (see Materials and Methods) and expressed as OD at 575 nm. Panel B: Macrophages were incubated as described above and NO production measured as concentration of nitrite in the culture media as described in Materials and Methods. Mean values not sharing a common letter are significantly different (p < 0.05).