Figure 5
From: Isolation of bacteria-containing phagosomes by magnetic selection
Phagosome analysis. Analysis of 15-min phagosomes, purified using the protocol for heat-killed bacteria. In A, immunofluorescence analysis of phagosomes is exemplified. Arrows show free bacteria, arrowheads intact phagosomes, double arrowheads broken phagosomes, and the dotted circle represents an intact phagosome negative for CD63 staining; scale bar 10 μm. Panel B shows Western blot analysis. Phagosomes (5·106) and varying amounts of whole-cell lysates (4·104, 1·105, 2·105, and 4·105) were probed with an antibody against myeloperoxidase (MPO). Two mature-form fragments (MPOlarge and MPOsmall) and a precursor form (proMPO) can be detected.