Kindlin-2 expression during myogenesis in vitro. Western analysis of kindlin-2 on C2C12 cell protein extracts. C2C12 cells we cultured in either growth media (GM) or differentiation media (DM) for 1, 2, 3, or 4 days. Kindlin-2 levels were compared to an early (myogenin) and a late (myosin heavy chain; MHC) marker of differentiation. GAPDH was used as a loading control. Kindlin-2 expression was induced upon differentiation, with a peak expression at day 3 post-differentiation. Of note, the GM cell extract was from confluent cells, and confluence stimulates a small amount of differentiation.